Explanation: CRISPR-Cas9 is most commonly known to be a gene editor, but since its initial discovery, people have found that with some minor tweaks, CRISPR can be made to do a bunch of other things, things that biologists could have only dreamed of previously.

If you introduce a mutation that deactivates the Cas9 protein, the entire CRISPR complex still binds to DNA. Then, you can essentially glue whatever you want onto this modified Cas9 to produce a bunch of different effects:

• deactivated Cas9, no additional modifications: blocks other proteins from touching the DNA, which suppresses a gene without destroying it
• glue on some transcriptional activators: makes the cell express a gene
• glue on some fluorescent proteins: makes your DNA glow, lets you see where it is

I struggled with your exact same problem when I moved to Linux. For the record, the reason why there isn’t a Program Files x86 is because the way that Linux stores programs is different from the way that Windows stores programs. Windows sorts its system files by programs, so that the configuration, launcher, and software files are all together. Linux sorts by file type, so that the configuration files of all programs are together, the launchers of all programs are together, etc.

What I’ve come to learn is, don’t bother trying to find your application in the folder. The best way to install things is through Discover (or whatever GUI software manager you’re using). If the program you want isn’t there, it’s basically a crapshoot whether installing from the internet will work or not

The Sun god demands more sacrifices

No, unless you already know how to pilot the ship, playing in VR is probably going to get you severely motion sick

I’m unsure what you mean by switches, since all mechanical keyboards use switches.

Regardless, if you’re looking for a relatively cheap, good quality mechanical keyboard, I’d probably recommend you check out Keychron. They’ve got a lot of options and they’re all decent quality for a decent price, so you could probably find something you want in their catalog

Fwiw that qPCR curve doesn’t look that bad, just don’t have the y-axis be in log form

Can she make a law that she cannot break? Checkmate Christians

Funny you bring up eel reproduction. Had to share. https://youtu.be/TzN148WQ2OQ

This is a map of signaling pathways in a cell. Essentially, how does a cell detect a stimulus and how does the cell respond to it. Signaling networks are notoriously difficult to parse, in major part because many of the proteins that make up the network are promiscuous (meaning that they target many types of proteins instead of just 1). Think of network as less of a series of defined steps and more of a chaotic mess. It’s so chaotic that most maps will select a theme (ie, hormone detection, stress signaling, infection, etc.) and only show the interactions that are relevant to that theme. Unfortunately, that also means that each map will show a network that’s completely different from the others, even though the proteins are the same.

The joke here is that it’s very tempting to make another map. This map is finally going to solve cell biology, I promise, we just have to make another map. Ignore the fact that we already have so many maps, just one more map is all we need

First, make sure you’re never alone with her. It’s important to have someone be able to vouch for you that nothing bad happened. Even if it’s not her intention, rumors spread and mutate in the most unexpected ways, and you’ll want to make sure you’re in the clear if it ever comes to pass that people start to suspect that you are having a relationship with her.

Second, you’ll want to speak with her. Make it clear that you are serious and that this is a serious talk. Whenever you see her next, just say something like “we need to have a talk.” Might be cliche, but the cliche aspect of it reinforces the serious nature of it IMO, since most people already associate that phrase with something serious.

You don’t need to be rude, but you need to be very clear where you stand on the matter. Make it clear that 1) you are not in a relationship with her, 2) you do not wish to be in a relationship with her, 3) you don’t appreciate her saying that you’re in a relationship with her. With talks of this nature, there’s no point beating around the bush. She may be temporarily hurt, but there is a difference between being hurt due to rejection and hurt due to injustice. She will come to appreciate the honesty in time.

The thought has never crossed my mind, and it would have never crossed the minds of anyone I know. I have no urge to cheat and neither does my partner. I have generally thought that it’s a very unusual and uncommon phenomenon for someone to cheat, and I find it surprising how many people here think otherwise

This is an unnecessarily rude comment that just proves that the parent comment was right in saying that you’re an unpleasant person. I hope you reconsider what behavior you consider to be acceptable

In the book, it’s fairly clear that he did kill everyone but only got away with it because 1) nobody can tell anybody else apart because everyone looks the same and 2) everybody is so self-centered that even if they did know a murder took place, they didn’t bother to report it.

It’s very heavily implied in the book that the landlady covered up Paul Allen’s (and multiple other prostitutes’) murder, presumably to maintain the property value of the apartment. Unfortunately, a lot of the clues don’t translate well into a movie:

1. The apartment only recently became available to rent
2. Bateman notes that the paint on the walls is still fresh, barely dried
3. Bateman notes that there’s a lot of potpourri laid out, as though to cover up a smell
4. Iirc the furniture was also brand new
5. The landlady immediately knew who Bateman was and asked him to leave

Chemistry might not be much better. It’s because scientists generally assume that readers already know how to do the techniques, and so the only information they would care to provide are the ones that wouldn’t be considered obvious. Such as equipment brand, the name of the technique if there’s multiple techniques that do the same thing, or experiment-specific modifications to the technique.

My understanding is that it’s a holdover from older times, when scientists were charged per word, and so methodology would be cut down to remove anything considered “general enough” knowledge

If you’re asking scientists about writing protocols, you clearly don’t know how scientific protocols work. If anything, scientists need to take lessons from recipe writers on how to write protocols. Scientific protocols are notoriously difficult to replicate.

Here’s a burger recipe written like a scientific methodology:

Raw beef patties (Carshire Butcher) were prepared on a grill (Grillman) according to manufacturer’s instructions. The burger was assembled with the prepared patties, burger bun (Lee Bakery), lettuce (Jordan Farms), American cheese (Cairn Dairy), and various toppings as necessary. Condiments were used where appropriate. Assembled burgers were served within 15 minutes of completion.

Reference to an Aesop fable. In the original version, the scorpion stings the frog and both of them drown. The moral is that some people are intrinsically harmful, even against their own self interest

OnlyOffice. Newer and somewhat less features, but the UI is so much better than LibreOffice. I’ve been on a quest to find the best Office alternative and OnlyOffice is what I’ve settled on

Distilled water still has impurities, just not the kind that will leave a residue

I personally prefer way the things they are now (show downvotes). I’m not quite sure what the reasoning would be to hide downvotes on a user’s own posts?